-- select by manufacturer -- ADAM EQUIPMENT ALLA FRANCE ANGELANTONI Burkle GmbH ECOSAFE FILTRA FUNKE–GERBER GVS Group HEINZ – HERENZ HELLMA ILMVAC INTERSCIENCE KARL HECHT ASSISTENT KARTELL KOEHLER INSTRUMENT KONIK-TECH Lab M MESA LABS MIELE MUNKTELL AHLSTROM NUOVA APTACA Orum International PBI INTERNATIONAL PRENTEX SAINT-GOBAIN Performance Plastics SELECTA SILVERSON Smeg instruments STABLE MICRO SYSTEMS SYNBIOSIS TEKNOKROMA WASSERLAB WHATMAN Whirl-Pak Lamo
Select by name - TYGON and other types of special hoses and tubes from Saint-Gobain. SIMAS supplier. Autoclaves, manual and automatic. Catalog SIMAS (SELECTA) Autoclaves. Brochure SIMAS (PBI INTERNATIONAL) Automatic media cookers and media preparation stations. Brochure SIMAS (PBI INTERNATIONAL) Automatic thinner Baby Gravimat. Brochure SIMAS (INTERSCIENCE) Automatic thinner Gravimat. Brochure SIMAS (INTERSCIENCE) Automatic washing and disinfection machines. Miele catalogue. Supplier SIMAS. Accessories for weighing and analytical equipment. Brochure SIMAS Accessories for gas chromatography. Brochure SIMAS (TEKNOKROMA) Accessories for ProtoCOL2. SYNBIOSIS Brochure. Supplier SIMAS. Analysis of aromatic hydrocarbons in petroleum products by HPLC+GC-MS. Catalog SIMAS (KONIK-TECH) Analysis of fatty acids in food products using a gas chromatograph and mass spectrometer KONIK. Catalog SIMAS (KONIK-TECH) Analysis of egg quality using a Texture Analyzer. Stable Micro Systems Brochure. Supplier SIMAS. Analysis of PAHs in mineral oils by HRGC+HPLC. Catalog SIMAS (KONIK-TECH) Texture analyzers for the food industry. Stable Micro Systems Brochure. SIMAS supplier. Texture analyzers for pharmaceuticals. Stable Micro Systems Brochure. Supplier SIMAS. Analytical laboratory. Catalog SIMAS (SELECTA) Analytical instruments for water and food analysis. Catalog SELECTA. Supplier SIMAS Analytical instruments. Brochure SIMAS (SELECTA) Analytical and general laboratory equipment. Catalog SELECTA 2011-2013. Analytical and general laboratory equipment. Catalog SELECTA 2015. SIMAS supplier. Analytical equipment for beer. Catalog SIMAS (FUNKE - GERBER) Basic model of torque indicator ST-FT1 Germreduc closed-type bactericidal installations. Brochure SIMAS (PBI INTERNATIONAL) Safety tanks and cans Safety containers, cans Biological and microbiological instruments and accessories. Catalog SELECTA. Supplier SIMAS Gemini box with vertical laminar air flow. Brochure Angelantoni. Supplier SIMAS Biological safety cabinets, laminar flow cabinets for equipping individual workplaces, working with powders. Brochure Angelantoni. SIMAS supplier More than filtering. Catalog WHATMAN Schleicher & Schuell. Supplier SIMAS Paper for testing seed germination. Munktell Brochure. Supplier SIMAS. Paper for collecting blood samples from newborns for research. Munktell Brochure. SIMAS supplier. Chromatography and blotting paper from Munktell. Supplier SIMAS. Coated paper to protect surfaces. Munktell Brochure. SIMAS supplier. Paper and membrane filters for the brewing and beverage industry. Munktell Brochure. Supplier SIMAS. Vacuum and high temperature cabinets. Brochure SIMAS (SELECTA, ILMVAC) Vacuum units and accessories for solid phase extraction. Catalog TEKNOKROMA. Supplier SIMAS Scales and Density Meter. Catalog SELECTA. Supplier SIMAS Electronic laboratory scales PGW, analytical electronic scales PW. Brochure SIMAS Rotational viscometers FUNGILAB. Catalog SIMAS 2009. Viscometers. Ford Cup. Brochure SIMAS (SELECTA) Water, oil and sand baths. Catalog SELECTA. Supplier SIMAS Pinocchio Super air intake for compressed air and gases. Brochure SIMAS (PBI INTERNATIONAL) Air intake SAS ISOLATOR for monitoring air purity in isolators and clean rooms. Brochure SIMAS (PBI INTERNATIONAL) Built-in high shear mixers. Catalog SIMAS (SILVERSON) High-quality products for laboratories - rubber, plastic, silicone High-precision refrigerated cabinets Hotcold. Brochure SIMAS (SELECTA) Highly efficient sample preparation for chromatography and mass spectrometry. Brochure SIMAS (WHATMAN) High-efficiency mixing equipment SILVERSON 2009. High-efficiency laboratory mixers SILVERSON. Catalog 2009. Safehood fume hood. Brochure BIOAIR. Supplier of SIMAS HPLC, HPLC, MS. Catalog SIMAS (KONIK-TECH) Gas chromatograph KONIK GC 4000 B. Catalog SIMAS (KONIK-TECH) Helios C - laminar box with horizontal air flow. Brochure Angelantoni. Supplier SIMAS General catalog KARTELL (plastic tableware). Supplier SIMAS HOMOGENIZER "PROBLAND" Hospital supplies. Catalog SIMAS (PBI INTERNATIONAL, KARTELL) Gravimetric diluent PRODELYUTE Deodorant for autoclaves - ANABAC capsules. Brochure SIMAS (INTERSCIENCE). Deodorant for autoclaves ANABAC. Brochure SIMAS (INTERSCIENCE) Liquid chromatography. Speakers and accessories. Catalog TEKNOKROMA. Supplier SIMAS Liquid and gas chromatographs KONIK K2. Catalog SIMAS (KONIK-TECH) Liquid chromatograph KONIK LC550 B. Catalog SIMAS (KONIK-TECH) Protective microbiological cabinet S@feflow. Brochure BIOAIR. SIMAS supplier Microbiological safety cabinet S@feflow 1.2. Brochure BIOAIR. Supplier SIMAS TopSafe microbiological safety cabinet. Brochure BIOAIR. Supplier of SIMAS Indicator and test paper. Catalog MACHEREY NAGEL. Supplier SIMAS Flow indicators, adapters and taps. Brochure SIMAS (KARTELL) Indicators, biological and chemical. Brochure SIMAS (MESA LABS) Automated instrument ChromaZone Medical instruments. Brochure SIMAS (NUOVA APTACA) Study of anti-corrosion properties of oils GOST 19199. Brochure SIMAS (SELECTA, WHATMAN) Study of petroleum products. Brochure SIMAS (KOEHLER INSTRUMENT) Research of petroleum products. Catalog SIMAS (KOEHLER) New items 2008 Research of petroleum products. Catalog SIMAS (KOEHLER,THE TINTOMETER, SELECTA) Canisters and containers for flammable liquids. Brochure SIMAS (SELECTA) Capillary columns for liquid chromatography. Catalog SIMAS (Teknokroma) Catalog Orum International New generation for microbiological air monitoring Biological air samplers Catalog - ALLA FRANCE Catalog - KARTELL - Laboratory supplies Catalog - Laboratory instruments for hospitals Catalog Heinz Herenz 2009. Medical and laboratory supplies. NASCO Catalog - Whirl-Pak Packages WHATMAN Catalog - leader in separation technology. SIMAS Supplier Whatman Catalog 2009-2010 – Touching Life. SIMAS supplier. Catalog of culture media LAB M. Supplier SIMAS. Whatman Catalog Book No. 1 - 1000 Little Things for the Laboratory Book No. 1 - Biological Air Samplers Book No. 10 - Soxhlet Extraction Book No. 11 - High Efficiency Mixers - Laboratory, Pilot and Industrial Book No. 12 - Cleaning and Disinfecting Laboratory Glassware Book No. 2 - Innovation for the microbiological laboratory Book No. 2 - Pumps and samplers Book No. 3 - Determination of flash points and self-ignition. Petrochemical series Book No. 3 - Modern chemical laboratory Book No. 4 - Study of milk and beer Book No. 5 - Vacuum and pressure filtration Book No. 6 - Study of the properties of petroleum oils - Petrochemical series Book No. 6 - Microbiological diagnostics of water, drinks and solutions Book No. 7 - Study of the plastic properties of lubricants. Petrochemical series Book No. 7 - Modern microbiological laboratory Book No. 8 - Study of rheological properties - Texture analyzers Book No. 8 - Study of rheological properties. Texture analyzers. Series "Laboratory Library". Book No. 9 - Texture analysis of industrial products Book No. 11 - High-efficiency mixers. Laboratory, pilot and industrial. Series "Laboratory Library". Book No. 12 - Washing and disinfecting laboratory glassware. Series "Laboratory Library". Book No. 2 - Pumps and samplers. Series "Laboratory Library". Book No. 3 - Modern chemical laboratory. Series "Laboratory Library". Book No. 4 - Research on milk and beer. Series "Laboratory Library". Book No. 5 - Vacuum and pressure filtration. Series "Laboratory Library". Book No. 6 - Study of the properties of petroleum oils. "Laboratory library. Petrochemical series." Book No. 6 - Microbiological diagnostics of water, drinks and solutions. Series "Laboratory Library". Collection of filter attachments. WHATMAN Catalog (BATMAH). Supplier SIMAS Columns for gas chromatography. Teknokroma catalogue. Supplier of SIMAS Colorimeters, photometers and spectrophotometers. Catalog SELECTA. SIMAS supplier Compact fume hood Chemo-Bench2 for chemical laboratories. Brochure SIMAS (PBI INTERNATIONAL) Conductivity and pH meters. Catalog SELECTA. Supplier SIMAS Containers and containers for waste collection. Brochure SIMAS (NUOVA APTACA) Cuvettes for spectrophotometry. Catalog HELLMA 2009.BestCellers. Cuvettes. Catalog SIMAS (HELLMA GmbH&Co.KG.) Laboratory furniture and equipment. SIMAS supplier. Laboratory products for the development and control of modern high-precision technologies and scientific research from WHATMAN. SIMAS catalog 2009. Laboratory equipment #1. Brochure SIMAS (SELECTA) Laboratory equipment #2. Brochure SIMAS (SELECTA) Laboratory equipment #3. Brochure SIMAS (SELECTA) Laboratory equipment for milk analysis. Catalog FUNKE-GERBER. Laboratory instruments and accessories. Catalog SELECTA. Supplier SIMAS Laboratory paddle mixers. Brochure SIMAS (BUNSEN) Laboratory devices for medical institutions No. 1. Brochure SIMAS (SELECTA) Laboratory devices for medical institutions No. 2. Brochure SIMAS (SELECTA) Laboratory paddle mixer Bag Mixer 400. Brochure SIMAS (INTERSСIENCE) Laboratory water purifier Micromatic Laminar flow cabinet II protection class SPACE2 ergosafe. Brochure SIMAS (PBI INTERNATIONAL) Laminar flow cabinet MINIFLO. Brochure SIMAS (PBI INTERNATIONAL) Laminar flow cabinet of protection class II SPACE Luce. Brochure SIMAS (PBI INTERNATIONAL) Vane homogenizer MiniMix 100 CC. Brochure SIMAS (INTERSCIENCE). Magnetic stirrers and rockers. Catalog SELECTA. Supplier of SIMAS Lubricating oils and additives. Method for determining the degree of purity GOST 12275. Brochure SIMAS (WHATMAN, SELECTA, ILMVAC) Mass spectrometer KONIK MS Q12. Catalog SIMAS (KONIK-TECH) Medical ultra-low temperature freezers. Brochure Angelantoni. Supplier SIMAS Membrane filtration for microbiology laboratories from Munktell. SIMAS supplier. Microbiological monitors are ready-made systems for sample preparation of liquid samples. Brochure SIMAS (WHATMAN) Microbiological products from PBI. Supplier SIMAS Microbiological control of water and beverages. Catalog SIMAS (WHATMAN) Microbiology - 2004 - PBI International Microbiology. Interscience Catalog 2011. Supplier SIMAS Microbiology. Interscience Catalog. Supplier SIMAS. Microbiology. Catalog WHATMAN Schleicher & Schuell. Supplier SIMAS Microbiology: dilution, homogenization, spiral inoculation, automatic colony counting, autoclaving. Catalog SIMAS (INTERSIENCE) Multifunctional laboratory mixers. SILVERSON MACHINES Catalog. Muffle furnaces. Catalog SELECTA. Supplier SIMAS Heating plates and heaters for round bottom flasks. Catalog SELECTA. Supplier SIMAS Vacuum, peristaltic, centrifugal pumps. Catalog SELECTA. Supplier SIMAS Pumps, fillers, samplers. Burkle catalog 2010-2011 in Russian. ST Benchtop Torque Tester Protran Nitrocellulose Membranes for the latest protein testing methods. Brochure SIMAS (WHATMAN) New catalog of Burkle 2010 in Russian. Pumps, samplers, containers, laboratory plastic, fittings (tubes, connections, clamps). Cellulose-based carriers for chromatographic columns and TLC. Brochure SIMAS (WHATMAN) Equipment for milk analysis. Catalog FUNKE GERBER. Supplier SIMAS Equipment and consumables for PCR and ELISA laboratories. SIMAS Brochure. Dispo-Head Sterile Dispo-Head for SAS Air Intakes. Brochure SIMAS (PBI INTERNATIONAL) Disposable serological pipettes. Kartell catalogue. Supplier SIMAS Ashing - muffle furnaces, crucibles, tongs. Brochure SIMAS (SELECTA) Determination of water in petroleum products GOST 2477. Brochure SIMAS (SELECTA, WHATMAN) Determination of viscosity of petroleum products. Brochure SIMAS (KOEHLER INSTRUMENT) Determination of viscosity. Brochure SIMAS (FUNGILAB, SELECTA) Determination of naphthenic acid soaps in fuels GOST 21103. Brochure SIMAS (WHATMAN, SELECTA, KARTELL) Determination of sediment in crude oil by membrane filtration method ASTM D4807-05. Brochure SIMAS (WHATMAN, SELECTA, ILMVAC) Determination of the content of mechanical impurities in petroleum products. GOST 10577. Brochure SIMAS (WHATMAN, ILMVAC) Optics: microscopes, refractometers, microtome. Catalog SELECTA. SIMAS supplier Sampling: solid, granular, pasty, liquid. SIMAS Brochure (PBI INTERNATIONAL) Torqo-II+ Torque Force Assessment SSB Bags - Ultra-Safe Sample Transport System. Brochure SIMAS (NASCO) Sto-Circul-Bag for homogenization. Brochure SIMAS (PBI INTERNATIONAL) VORTEX packages - sampling, packaging, transportation, homogenization and storage of samples. SIMAS catalog (NASCO, INTERSCIENCE, PBI INTERNATIONAL) VORTEX packages for industrial enterprises. Brochure SIMAS (NASCO) Portable unit for disinfection of premises Formalintec. Brochure SIMAS (PBI INTERNATIONAL) Portable automatic autoclave Auto-Koch. Brochure SIMAS (PBI INTERNATIONAL) Ovens, sterilizers, incubators and drying ovens. Catalog SELECTA. Supplier SIMAS Culture media LAB M. Complete list 2012. Supplier SIMAS. Culture media for microbiological research in clinical microbiology and food control from Lab M. Brochure SIMAS. Plastic glassware for laboratories. Kartell 2012. SIMAS supplier. Flexible TLC plates. Catalog SIMAS (WHATMAN) Diamond series TLC plates. Catalog SIMAS (WHATMAN) Immersion thermostats. Catalog SELECTA. SIMAS supplier Preparation of groundwater samples using Polydisc GW. Brochure SIMAS (WHATMAN) Sample preparation for HPLC - Mini-Uni Prep piston filter elements. Brochure SIMAS (WHATMAN) Sample preparation for chromatographic and spectrometric studies. Brochure SIMAS (WHATMAN) Colony counting with Petri-Light and Colony Count instruments. Brochure SIMAS (PBI INTERNATIONAL) Polymer containers. Catalog SIMAS (KARTELL) Polymer products for laboratories No. 1. Brochure SIMAS (KARTELL) Polymer products for laboratories No. 2. Brochure SIMAS (KARTELL) Polymer products for ultra-clean and aggressive environments from Saint-Gobain. SIMAS supplier. Polymer bags for autoclaving DISPOBAG. SIMAS Brochure (PBI INTERNATIONAL) Complete line of KONIK-TECH products 2007 SIMAS Catalog. SMS device in the magazine Food Industry No. 3 2019 SMS device in the magazine Food Industry No. 4 2019 Auxiliary devices for testing petroleum products Devices for analyzing petroleum products. Catalog KOECHLER. Supplier SIMAS Instruments for monitoring and control. Catalog SELECTA. Supplier SIMAS Stoppers. Brochure SIMAS (KARTELL) Air sampler AIRBIO DUO Air sampler SYSTEM KALITEST Air sampler TRIO.BAS TRIO Sampler for analysis of aerosols for legionella SAS-PCR LEGIONELLA. Brochure SIMAS (PBI INTERNATIONAL) Food samplers. Brochure SIMAS (PBI INTERNATIONAL) Sales program SIMAS Washers. Brochure SIMAS (KARTELL) Simple colony counting and measurement of inhibition zones. SYNBIOSIS Brochure. SIMAS supplier. Dilution and homogenization of samples for microbiological studies. Brochure SIMAS (INTERSCIENCE) Sample sieving. Filtra catalog. Supplier SIMAS Sample sieving: devices and sieves. Brochure SIMAS (FILTRA) Sieving Filtra catalog 2011. Supplier SIMAS. Consumables for blood tests, auxiliary laboratory equipment. Catalog SELECTA. Supplier SIMAS Microbiology consumables. Brochure SIMAS (NUOVA APTACA, HEINZ - HERENZ, KARL HECHT) Consumables for optical analytical methods. research. Catalog 2011 Supplier SIMAS. Consumables and accessories. Catalog HEINZ-HERENZ. Supplier of SIMAS Consumables, accessories, equipment for microbiologists. SIMAS catalog (HEINZ – HERENZ, KARTELL, NASCO, PBI INTERNATIONAL, SELECTA, SAINT-GOBAIN Performance Plasticks) Hand-held tester ST-H0X Hand-held rotary pumps for all liquids. Brochure SIMAS (SELECTA) Air intake family. Brochure SIMAS (PBI INTERNATIONAL) SAS Super Network for Cleanrooms. Brochure SIMAS (PBI INTERNATIONAL) ProtoCOL 2 system for: colony counting, zone measurement. SYNBIOSIS Brochure. Supplier SIMAS System GAS - TEST Water purification systems Wasserlab Sieves and diffusers. Catalog FILTRA. SIMAS supplier Modern products for laboratories, industry and science. SIMAS catalog (BURKLE 2003) Modern products for laboratories, industry and science. Catalog SIMAS (BURKLE 2007) Laboratory glassware, microscopy, accessories. Catalog Assistent-Precision. Glass products and accessories. Catalog ASSISTENT (KARL HECHT KG). Supplier SIMAS Sterile cellulose stoppers for microbiology. Brochure SIMAS (HEINZ – HERENZ) Dry thermostats. Catalog SELECTA. Supplier SIMAS Colony counter Acolyte. SYNBIOSIS Brochure. Supplier SIMAS. Colony counter SCAN 100. Brochure SIMAS (INTERSCIENCE). Automatic colony counter PROTOCOL 3 Table of correspondence between TEKNOKROMA columns and columns from other manufacturers. Supplier of SIMAS Thermometers, hydrometers for all types of industry. Catalog 2011. Supplier SIMAS Thermometers, hydrometers, refractometers for the food industry. Catalog 2008 Russian language. Supplier SIMAS Test for determining surface cleanliness IGIENTEST. SIMAS leaflet. Filtration and separation technologies. Munktell catalog 2012. WATMAN - Filter GDX Ultrasonic cleaning baths. Catalog SELECTA. Supplier SIMAS Installation MBS -I vacuum membrane filtration for the food industry. Brochure SIMAS (WHATMAN) MBS-II vacuum membrane filtration unit for the pharmaceutical industry. Brochure SIMAS (WHATMAN, ILMVAC) Installation for automatic filling of Petri dishes Elios Premium. Brochure SIMAS (PBI INTERNATIONAL) Installation for thermal sealing of Bag Seal packages. Brochure SIMAS (INTERSCIENCE) Filter paper Munktell & Filtrak. Supplier SIMAS. Filter paper for quantitative analysis. Munktell Brochure. Supplier SIMAS. Filter paper for chemical laboratories in the food industry and agriculture from Munktell. SIMAS supplier. Filter paper brand FILTRAK. Munktell Brochure. Supplier SIMAS. MBS I filter system for microbiological control. Brochure SIMAS (WHATMAN) MBS II filter system for microbiological control. Brochure SIMAS (WHATMAN) GD/X syringe filter attachments for viscous liquids. Brochure SIMAS (WHATMAN) Paper and fiberglass filters, extraction bushings. Brochures SIMAS (WHATMAN)-(BATMAH) Refrigeration and freezing units. Catalog SELECTA. Supplier of SIMAS Chromatography for the dairy industry. Supplier SIMAS Chromatography. Teknokroma catalog 2009-2010. SIMAS supplier. Cellulose extraction thimbles for Soxhlet extraction. Munktell Brochure. SIMAS supplier. Centrifuges. Catalog SELECTA. SIMAS supplier Safety cabinets - 1 Safety cabinets - 2 Safety cabinets - 3 Safety cabinets - 4 Phthalate-free hoses. Catalog Saint-Gobain. Supplier SIMAS. Tygon hoses and tubes. Application. SIMAS supplier. Hoses and tubes TYGON. Technical specifications. SIMAS supplier. Hoses. Brochure SIMAS (KARTELL) Rack for storing bags for homogenization BagRack 400. Brochure SIMAS (INTERSCIENCE)
Compound**:
** The composition has been verified and adjusted to meet the required parameters
Preparation:
Mix 45.6 g of M304 powder or 30.6 g of M303 powder in 1000 ml of distilled water. Heat to a boil to completely dissolve the particles. If the medium is to be used the same day, autoclaving is not required. Otherwise, sterilize by autoclaving at 0.9 atm (118°C) for 15 minutes. DO NOT OVERHEAT THE ENVIRONMENT.
Warning: Sodium azide tends to form explosive compounds with metals, so it is recommended to use plenty of water to remove any remaining media.
Principle and evaluation of the result:
These media are based on the Pike recipe (1) proposed for the selective isolation of streptococci from various materials, especially those heavily contaminated with accompanying microflora (2). Other authors (3) have shown the possibility of using these media for isolating group A b-hemolytic streptococci.
Papain digest of soy flour, casein hydrolyzate, salts and glucose serve as a source of essential nutrients for the growth of streptococci. Sodium azide and sulfite inhibit the growth of gram-positive rods, and crystal violet inhibits the growth of staphylococci. At the same time, these inhibitors in these concentrations do not suppress the growth of streptococci, so the media can be used for isolating and cultivating streptococci in sanitary-epidemiological and clinical studies. This medium actively inhibits the growth of coliform bacteria, Proteus, pseudomonads and bacilli, but some staphylococci and pneumococci can grow on it. All streptococcal colonies grown on this medium require further identification.
Quality control:
Powder appearance:
Homogeneous free-flowing yellow powder.
Density of the finished medium:
A medium is formed corresponding in density to a 1.5% agar gel (M304).
Color and transparency of the finished medium:
The medium is light amber in color, transparent or slightly opalescent if a gel forms in Petri dishes or test tubes.
Acidity of the environment:
At 25°C aqueous solutions M304 (4.56% w/v) or M303 (3.06% w/v) have a pH of 7.4 ± 0.2.
Cultural properties:
Growth characteristics of reference strains after 18-24 hours at 35-37°C.
|
Strains of microorganisms (ATSS) |
Height |
Owners of patent RU 2553224:
The group of inventions relates to biotechnology. Variants of the nutrient medium for the selective accumulation of enterobacteria contain pancreatic hydrolyzate of fishmeal, or meat peptone, or pancreatic hydrolyzate of fishmeal and meat peptone (in a ratio of 1:1), purified modified bile (PBM), sodium chloride, glucose, potassium phosphate monosubstituted and brilliant green in a given ratio. The inventions make it possible to increase the efficiency of the accumulation of enterobacteria, the selective properties of the medium and simplify the method of obtaining a nutrient medium. 3 n.p. files, 3 tables, 9 pr.
The invention relates to sanitary and clinical microbiology and can be used for the selective accumulation of enterobacteria from water, food products, clinical material, etc.
Leading foreign companies: Merck “Microbiology”, 2004-2005 p.202; History meat. Component content in g/l is presented in Table 1.
For bacteriological control in accordance with the State Pharmacopoeia of the Russian Federation, XII edition, which regulates the list of nutrient media for selective enrichment of enterobacteria, Mossel broth (Enterobacteria Enrichment Broth-Mossel) OFS 42-0067-07 is provided.
The selective accumulation of all types of enterobacteria in Mossel broth is due to the fact that a high concentration of ox bile in combination with brilliant green suppresses the growth of accompanying microflora, and glucose promotes the growth of enterobacteria. Sodium-potassium phosphates provide a large buffer capacity in the medium, which protects the culture from the harmful effects of the resulting acid.
Until now, in Russia, due to the lack of a domestic commercial dry nutrient medium for the selective enrichment of enterobacteria in the practice of clinical and sanitary microbiology, laboratory-prepared Mossel broth is used, and to control the microbiological purity of medicinal media - medium No. 3 (enrichment medium for bacteria of the family Enterobacteriaceae).
The disadvantages of laboratory-made Mossel broth in accordance with the recipe presented in the Global Fund, XII edition, and medium No. 3 are:
Difficulty of preparation;
The use of unpurified cattle bile as an selective factor - a substance of chemically uncertain composition that makes it difficult to obtain objective results;
Lack of domestic dry preparations - peptone and bile, which have a high degree of transparency in solutions;
The need for filtration in the case of using the main components of the domestically produced medium, as an additional step in the preparation process;
Weak inhibitory ability against accompanying gram-positive microbe associates while ensuring the growth of enterobacteria;
Increased cost of the environment if it is necessary to use imported drugs.
The closest to the proposed Mossel broth is commercial Mossel broth (Merck), which consists of the following components, g/l:
The disadvantage of a commercial environment (Merck) is:
Insufficient efficiency in the growth and selective accumulation of a number of enterobacteria;
Insufficiently expressed inhibitory properties against associated microbes;
Limited availability.
The objective of the invention is to create a domestic dry nutrient medium for the selective accumulation of enterobacteria, which is more efficient, with higher selective properties, ensuring accessibility and obtaining objective results of bacteriological control.
The problem is solved by the fact that a nutrient medium for the selective accumulation of enterobacteria is proposed, dry, containing a protein base, glucose, potassium phosphate monosubstituted and brilliant green, and as a protein base it contains pancreatic hydrolyzate of fishmeal, or meat peptone, or pancreatic hydrolyzate of fishmeal and meat peptone (in a 1:1 ratio), purified modified bile (PBM) and sodium chloride in the following quantitative ratio of components, g/l:
Option one:
Option two:
Option three:
The technical result is achieved through preliminary preparation of raw bile. For this purpose, native cattle bile is boiled and filtered through a filter cloth (belting) at isoelectric points, at each pH value, in order to precipitate proteins and high-molecular peptides. The final stage of bile preparation consists of adsorption of impurities by active carbon, adding the calculated amount of disubstituted sodium phosphate in terms of dry matter content, boiling and filtration. This eliminates the additional addition of sodium phosphate to the medium, providing a high buffer capacity ready environment and its transparency. The balance of the component composition of the medium, including pancreatic hydrolyzed fish meal (PGHM), as a protein nutrient base, ensures good growth and selective properties of Mossel broth.
The method for producing Mossel broth involves mixing dry ingredients, g/l:
Pancreatic hydrolyzate of fish meal or meat peptone provides the growth needs of microorganisms for nitrogen nutrition. Preliminary preparation of cattle bile makes it possible to obtain purified modified dry bile (PBM), which provides high buffer capacity and transparency of the finished nutrient medium, inhibitory properties against undesirable accompanying bacterial flora and selective accumulation of enterobacteria, which is not inhibited as a result of the formation of acidic waste products.
The difference between the proposed medium and the prototype is the possibility of using, along with meat peptone, pancreatic hydrolyzate of fish meal as a protein base. The technology for preparing raw bile using disubstituted sodium phosphate eliminates its additional addition to the medium. The quantitative ratio of the components of the nutrient medium, its pH, ensure that the medium maintains good growth properties, the efficiency of the accumulation of enterobacteria and the inhibitory effect on the accompanying microflora. The prepared medium remains sterile for at least 10 days
To achieve a technical result at the stage of production of purified modified bile (PBM), bile after precipitation of proteins at isoelectric points, adsorption of impurities by active carbon with the addition of dibasic sodium phosphate at the rate of 12.0 g for every 20.0 g of bile, in terms of dry matter, dried in a drying unit in a vibrating fluidized bed A1-FMU.
Example 1. To prepare the medium according to the first option, take weighed portions of the following ingredients, g/l:
The proposed medium, transparent, green in color, ensures the growth and accumulation of the following test strains of the Enterobacteriaceae family when inoculated with 0.5 ml of microbial suspension from a dilution of 10 -7:
E. coli O55:K59 3912/41
S. flexneri 1a 8516
S. typhimurium 79
K. pneumonia 418
K. pneumonia 3534/51
The proposed medium significantly inhibits the growth of gram-positive microorganisms, in particular S. aureus Wood-46, from a dilution of 10 -1.
The test strains of microorganisms used to control the environment were obtained from the department of collection cultures of the Federal Budgetary Institution of Science of the State Scientific Center for Medical and Biology.
Prepare a standard culture suspension of each test strain, corresponding to 10 units according to the standard turbidity sample OSO 42-28-85 P, using a sterile 0.9% sodium chloride solution. The resulting culture suspensions are diluted tenfold (4.5 ml of 0.9% sodium chloride solution with 0.5 ml of microbial suspension) brought to a dilution of 10 -7 (for S. aureus Wood-46 - to a dilution of 10 -1) and used for control environment.
To determine growth properties, inoculation is carried out with 0.5 ml of a microbial suspension of each test strain from a dilution of 10 -7 in 5.0 ml of Mossel broth, i.e. 10 m.c./ml medium. The crops were incubated at a temperature of (37±1)°C for 20-24 hours. The results of biological control of laboratory samples of Mossel broth on a set of test strains are presented in Table 2.
Example 2. The medium is prepared in accordance with example 1.
Example 3. The medium is prepared in accordance with example 1.
The results of biological control are presented in Table 2 and are similar to the results of testing in example 1.
Example 4. To prepare Mossel broth in accordance with the second option, take weighed portions of the following ingredients, g/l:
The samples are stirred in 1 liter of distilled water, poured into 5 ml test tubes and sterilized by autoclaving at 121°C for 5 minutes.
Example 5. The medium is prepared in accordance with example 4.
The results of biological control are presented in Table 3 and are similar to the results of testing examples 1, 2, 3 according to option 1.
Example 6. To prepare Mossel broth in accordance with the third option, take weighed portions of the following ingredients, g/l:
The samples are stirred in 1 liter of distilled water, poured into 5 ml test tubes and sterilized by autoclaving at 121°C for 5 minutes.
The results of biological control of Mossel broth in accordance with option 3 are presented in table 3 and are similar to the results of testing in examples 1, 2, 3.
Example 7. The medium is prepared in accordance with example 6.
The results of biological control are presented in Table 3 and are similar to the results of testing in examples 1, 2, 3.
Example 8. The medium is prepared in accordance with example 1.
The results of biological control are presented in Table 2.
Assessment of the quality of the proposed medium for the selective accumulation of enterobacteria with a violation of the quantitative ratio of ingredients:
Example 9. The medium is prepared in accordance with example 1.
The results of biological control are presented in Table 2. Violation of the quantitative ratio of the ingredients of the medium in examples 8, 9 leads to a deterioration in growth and inhibitory properties and, as a consequence, a decrease in the efficiency coefficient of the accumulation of enterobacteria.
From tables comparative characteristics biological control (Tables 2, 3) it is clear that the proposed nutrient medium in accordance with examples 1-7 has good growth properties, is effective in the accumulation of enterobacteria and inhibits the growth of staphylococcus.
Visual recording of the results of the accumulation of enterobacteria at the end of the incubation period of the crops showed identical results in the test and control media.
A change in the quantitative ratio of the components of the environment leads to a violation of the biological indicators of the quality of the proposed environment.
Thus, in the case of preparing the medium in accordance with examples 8, 9, a decrease in the efficiency of the accumulation of enterobacteria and the inhibitory effect of the medium against S. aureus Wood-46 is observed.
To obtain data on the effectiveness of Mossel broth, inoculated tubes of each test strain containing about 10 mc/ml - (zero inoculation) were incubated at a temperature of (37±1)°C for 3 hours. To determine the inoculum dose 0. 1 ml of microbial suspension of each test strain from a dilution of 10 -7 was sown on Petri dishes with GRM agar. After incubation of the crops in the accumulative medium, the contents of the tubes were mixed and a similar seeding was carried out on GRM agar.
After 18-20 hours of incubation of the crops on GRM agar at a temperature of (37±1)°C, the formed colonies were counted. The efficiency indicator was calculated by the increase in the number of colonies after incubation of the culture in an accumulative medium to the number of colonies with zero inoculation.
Comparative characteristics of the effectiveness of Mossel broth according to option 1 are presented in Table 3. The results of the efficiency of accumulation of enterobacteria in Mossel broth prepared in accordance with options 2 are similar and comparable to the results obtained when testing Mossel broth, option 1.
Thus, a domestic competitive dry nutrient medium has been developed for the selective enrichment of enterobacteria, which has a number of advantages:
the efficiency of accumulation of enterobacteria is 1.5-2.0 times higher than in a commercial environment;
the inhibition coefficient against staphylococcus is more than 100 times higher than that of a commercial enrichment broth;
the nutrient medium is easy to prepare;
a technology has been developed for clarification of bile at isoelectric points using activated carbon and disubstituted sodium phosphate, which eliminates its further addition to the nutrient medium and ensures the transparency of the finished product;
the need to use anhydrous sodium phosphate disubstituted in the manufacture of dry preparation is eliminated;
the possibility of using pancreatic hydrolyzate of fish meal along with meat peptone while maintaining growth and selective properties;
| Table 1 | |||||
| Accumulative broth for enterobacteria according to Mossel | |||||
| No. | Name of components | EO Mossel broth EE Broth, Mossel HiMedia | Enterobacterial broth enriched according to Mossel LLC "GEM" | Mossel broth for enrichment of enterobacteria (Enterobacteria Erichment Broth-Mossel OFS 42-0067-07) | |
| 1 | Pancreatic hydrolyzed gelatin | - | - | 10,0 | 10,0 |
| 2 | Meat peptone | 10,0 | 10,0 | - | - |
| 3 | Glucose monohydrate | 5,5 | 5,0 | 5,0 | 5,0 |
| 4 | Ox bile | 20,0 | 20,0 | 20,0 | 20,0 |
| 5 | Sodium phosphate disubstituted | 8,0 | 6,45 | 7,0 | 8,0 |
| 6 | Potassium phosphate monosubstituted | 2,0 | 2,0 | 3,0 | 2,0 |
| 7 | Diamond green | 0,015 | 0,0135 | 0,015 | 0,015 |
| pH of the prepared medium | 7.2±0.2 | 7.2±0.2 | 7.2±0.2 | 7.2±0.2 |
| table 2 | ||||||||
| Comparative characteristics of the biological control of Mossel broth based on pancreatic hydrolyzate of fishmeal, g/l | ||||||||
| No. | Name of test strain | Breeding | Option 1 | Option 1 | Option 1 | Option 1 | Option 1 | Mossel enrichment broth for enterobacteria (Mossel Broth) Merck |
| Example 1 | Example 2 | Example 3 | Example 8 | Example 9 | ||||
| 1 | E. coli 25922 | 10 -7 | +++ | +++ | +++ | ++ | ++ | +++ |
| 10 -6 | +++ | +++ | +++ | +++ | +++ | +++ | ||
| 2 | E. coli 055:K59 | 10 -7 | +++ | +++ | +++ | ++ | ++ | +++ sediment |
| 3912/41 | 10 -6 | +++ | +++ | +++ | +++ | +++ | +++ | |
| 3 | S. flexneri la 8516 | 10 -7 | ++ | ++ | ++ | + | + | ++ |
| 10 -6 | ++ | ++ | ++ | ++ | + | +++ | ||
| 4 | S. typhimurium 79 | 10 -7 | +++ | +++ | +++ | ++ | ++ | +++ |
| 10 -6 | +++ | +++ | +++ | +++ | ++ | +++ | ||
| 5 | K. pneumonia | 10 -7 | +++ | +++ | +++ | ++ | ++ | +++ |
| 418 | 10- 6 | +++ | +++ | +++ | +++ | ++ | +++ gas | |
| 6 | S. aureus-Wood-46 | 10 -1 | No diffuse turbidity of the medium | No diffuse turbidity of the medium | + weak diffuse turbidity of the medium | No diffuse turbidity of the medium | No diffuse turbidity of the medium | |
| Table 3 | |||||||
| Comparative characteristics of the biological control of Mossel broth based on meat peptone and a mixture of PGRM with meat peptone in a ratio of 1:1, g/l | |||||||
| No. | Name of test strain | Breeding | Option 2 | Option 2 | Option 3 | Option 3 | Mossel enrichment broth for enterobacteria (Mossel Broth) Merck |
| Example 4 | Example 5 | Example 6 | Example 7 | ||||
| 1 | E. coli 25922 | 10 -7 | +++ | +++ | +++ | ++ | +++ |
| 10 -6 | +++ | +++ | +++ | +++ | +++ | ||
| 2 | E. coli 055:K59 | 10 -7 | +++ | +++ | +++ | ++ | +++sediment |
| 3912/41 | 10 -6 | +++ | +++ | +++ | +++ | +++ | |
| 3 | S. flexneri la 8516 | 10 -7 | +++ | +++ | +++ | +++ | +++ |
| 10 -6 | +++ | +++ | +++ | +++ | +++ | ||
| 4 | S. typhimurium 79 | 10 -7 | +++ | +++ | +++ | +++ | +++ |
| 10 -6 | +++ | +++ | +++ | +++ | +++ | ||
| 5 | K. pneumonia | 10 -7 | +++ | +++ | +++ | +++ | +++ |
| 418 | 10 -6 | +++ | +++ | +++ | +++ | +++gas | |
| 6 | S. aureus-Wood-46 | 10 -1 | No diffuse turbidity of the medium | No diffuse turbidity of the medium | No diffuse turbidity of the medium | No diffuse turbidity of the medium | No diffuse turbidity of the medium |
| *+++ - excellent growth with diffuse turbidity of the medium; ++ - good growth with diffuse turbidity of the medium; |
1. Nutrient medium for the selective accumulation of enterobacteria, dry (Mossel Broth), containing a protein base, glucose, potassium phosphate monosubstituted and brilliant green, characterized in that it contains pancreatic hydrolyzate of fish meal as a protein base, additionally contains purified modified bile (POM) and sodium chloride with the following quantitative ratio of components, g/l:
2. Nutrient medium for the selective accumulation of enterobacteria, dry (Mossel Broth), containing meat peptone, glucose, potassium phosphate monosubstituted and brilliant green, characterized in that it contains purified modified bile (PBM) and sodium chloride in the following quantitative ratio of components, g /l:
3. Nutrient medium for the selective accumulation of enterobacteria, dry (Mossel Broth), containing a protein base, glucose, potassium phosphate monosubstituted and brilliant green, characterized in that the protein base contains pancreatic hydrolyzate of fish meal and meat peptone in a 1:1 ratio, additionally contains purified modified bile (PBM) and sodium chloride in the following quantitative ratio of components, g/l:
Similar patents:
The invention concerns a set of oligonucleotide primers and probes for identification and subtyping of DNA of the bacterium Pasteurella multocida serotypes A, B, D, E, F by real-time PCR.
The invention concerns a set of fluorescently labeled oligonucleotide probes for typing Burkholderia mallei strains. The probes included in the kit have a hairpin structure with a fluorophore and a fluorescence quencher at the ends and are complementary to the products of the amplification reaction with the primers.
The invention relates to food microbiology, in particular to the production of a nutrient medium for the determination of microorganisms that produce lipases in confectionery products.
The invention relates to medicine and can be used to identify urease-producing microorganisms, in particular Helicobacter pylori. To do this, a rapid analysis is carried out on diagnostic disks to determine the urease activity of the samples.
The group of inventions relates to biochemistry. A method for obtaining a standard sample of the turbidity of bacterial suspensions, a standard sample of the turbidity of bacterial suspensions, the use of a standard sample of the turbidity of bacterial suspensions, as well as a kit containing a standard sample of the turbidity of bacterial suspensions is proposed.
Sets of oligonucleotide probes and primers, a biological microchip and a test system for identifying and typing influenza A and B viruses are presented. The characterized test system contains: a set of reagents for isolating influenza virus RNA from human biological material; a set of reagents for carrying out PCR amplification combined with reverse transcription to form fluorescently labeled fragments of the influenza virus genome, containing the described oligonucleotide primers; a set of reagents for hybridization of DNA fragments obtained after amplification on a biochip containing elements with immobilized oligonucleotide probes described; and, if necessary, negative and positive controls.
The invention relates to biotechnology, in particular to determining the content of microorganisms in various objects and environments. The method involves the conjugation of bacteria with an electrochemical label, which is Fe0, MgFe2O4 or Fe3O4, carried out in an aqueous environment under specified parameters.
The invention relates to the field of medical microbiology and biotechnology, in particular to microbiological laboratory diagnosis of infectious diseases. The nutrient medium contains tryptone, yeast extract, glucose, sodium chloride, human erythrocytes 0(I) group Rh(+), iron(II) sulfate, Difco agar and distilled water at specified ratios of components.
The invention relates to biotechnology and can be used in bacteriological studies on the isolation and identification of bacteria of the genus Klebsiella, and the production of nutrient media for these studies.
A group of inventions related to the food and fermentation industry has been declared: cultures of kombucha Fungi Tea, Medusomyces gisevii alfa and Medusomyces gisevii, as well as a method for producing a fermented base for the production of kvass, a method for producing kombucha culture liquid and a method for producing drinks from vegetable juice or fermented vegetables .
The invention relates to biotechnology, microbiology and agriculture and can be used to obtain a bacterial preparation against plant diseases caused by phytopathogenic fungi of the genus Fusarium, Microdochium, Pyrenophora and Puccinia. Immunogenic composition for the treatment or prevention of clostridium difficile, a method for its preparation and a method for inducing an immune response to c. difficile // 2550271
An immunogenic composition is proposed for use in the treatment or prevention of diseases associated with Clostridium difficile, a method for obtaining such a composition by mixing its constituent ingredients, and a method for inducing an immune response to C.
The invention relates to the field of biotechnology. The Microbacterium species strain is deposited under registration number BKM Ac-2615D, used for the purification of brackish and marine water bodies. The invention provides enhanced destruction of naphthenic fractions, heterocyclic compounds and resins, as well as polyaromatic compounds, benzo(a)pyrene and benzo(a)anthracene in oil. 1 ill., 2 tables, 1 ex.
The group of inventions relates to biotechnology. Options for the nutrient medium for the selective accumulation of enterobacteria contain pancreatic hydrolyzate of fish meal, or meat peptone, or pancreatic hydrolyzate of fish meal and meat peptone, purified modified bile, sodium chloride, glucose, single-substituted potassium phosphate and brilliant green in a given ratio. The inventions make it possible to increase the efficiency of the accumulation of enterobacteria, the selective properties of the medium and simplify the method of obtaining a nutrient medium. 3 n.p. files, 3 tables, 9 pr.
Directions
Add 13.8 g of Bolton Broth to 500 ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25 ml Laked Horse Blood SR0048 and 1 vial of Bolton Broth Selective Supplement SR0183, reconstituted as directed. Mix well and distribute into sterile screw top containers.
Description
Bolton Selective Enrichment Broth is intended for the pre-enrichment of Campylobacter in food samples. Campylobacter are Gram-negative, spirally shaped microaerophilic organisms which may be present in raw milk, untreated water, improperly handled food and undercooked meats, poultry and shellfish. Human consumption of these organisms can result in a range of clinical illnesses from transient asymptomatic colonization to severe dysentery. The symptoms of Campylobacter enteritis include diarrhoea, stomach pain, nausea, fever, headache and muscle pain. Complications of infection by Campylobacter jejuni may include unnecessary appendectomies as a result of abdominal pain, reactive arthritis or Guillian-Barre syndrome 1 .
Campylobacter infection is recognized as one of the most common causes of bacterial gastroenteritis in humans, and the minimum infectious dose may be as low as 500-800 cells 1 .
Since awareness of the apparent role of Campylobacter in human disease was heightened by Skirrow in 1977 2, a great number of cultural media have evolved in response to the need to optimize performance. There was early recognition of the need for enrichment culture when examining food samples to overcome the damaging effects that food processing and preservation techniques can have on Campylobacter cells. Use of lower incubation temperatures in the early stages of enrichment is now widely established as an aid to cell recovery 3. This principle was employed by Bolton in the development of his enrichment broth 4.
Campylobacter can be injured by food processing and preservation procedures 3. This makes them susceptible to selective agents which are tolerated by undamaged cells. False negative results are avoided through use of recovery medium such as Bolton Selective Enrichment Broth which increases the number of cells available for culture, first by resuscitating injured organisms and then encouraging them to multiply.
Bolton Selective Enrichment Broth contains nutrients to aid resuscitation of sublethally injured cells, and is formulated to avoid the need for a microaerobic atmosphere. Initial incubation is carried out at 30-37°C, depending on the type of food to be examined. After the pre-enrichment, the incubation temperature is raised to 42°C to increase the selective pressures on competing organisms.
Inclusion of sodium metabisulphite and sodium pyruvate in Bolton Broth quenches toxic compounds that may form in the culture medium. These additions also increase the aero-tolerance of the culture. The antibiotics contained in Bolton Broth Selective Supplement SR0183 optimize selectivity for Campylobacter spp. Vancomycin - active against Gram- positives. Cefoperazone - predominantly active against Gram-negatives. Trimethoprim - active against a wide variety of Gram-negative and Gram-positive organisms. Cycloheximide - active against yeasts.
Technique
One method of use is as follows:
Place 25 g of food sample in 225 ml Bolton Selective Enrichment Broth (prepared as described above) and homogenize the mixture using a Stomacher (or similar device). Bolton Selective Enrichment Broth does not require incubation in a microaerobic environment, but must be used in screw topped containers which are filled to within 20 mm of the top 4 . Incubate for 4 hours at 37°C, followed by further incubation at 42°C. The broth can be subcultured after 24 hours and 48 hours on to either Modified CCDA (CM0739 + SR0155) or Preston Agar (CM0689 + SR0117 + SR0048) 4 .
For other methods please refer to BAM 5.
Storage conditions and Shelf Life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for up to 2 weeks.
Appearance
Dehydrated medium: Straw coloured, free-flowing powder.
Prepared medium: Broth: Straw colored solution containing small black particles.
Quality Control
